PTEN (also known as Posphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase, and dual-specificity protein phosphatase) is a member of the PI3K/PTEN/Akt pathway and a tumor suppressor. Defects of the antibody have been implicated in human cancers, including prostate, breast, thyroid, endometrium, skin, neck, brain, and head. Up to 60 percent of the advanced prostate cancers now show abnormal gene expression or a loss of the protein expression.
More Information
The PTEN antibody is for research purposes only and shouldn’t be used in diagnostic testing. It does have a clone called SP218, and the immunogen is the synthetic peptide derived from the human C-terminus of the protein. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 54 kDa.
Application/Procedure
You can use Immunohistochemistry, Flow Cytometry, or Westering Blotting with the PTEN antibody. For IHC procedures, you will need to use Formalin-fixed or paraffin-embedded tissues. When using the concentrate version, you should dilute the antibody using a ratio of one to 200. You can also find a pre-diluted version that works for IHC options, as well. To retrieve the antigen, you should boil tissue sections in a 10mM citrate buffer with a pH of 6.0 for at least ten minutes, allowing it to cool to room temperature for 20 minutes. Incubate it for 10 minutes at room temperature before visualization. The positive control for IHC procedures is the prostate or the prostate adenocarcinoma.
Western blotting procedures recommend diluting the antibody using a ratio of one to 400 and not using the pre-diluted formulation. Incubation periods are one hour at room temperature. The positive control here is the HeLa Cell Lysate.
The positive control for Flow Cytometry is the A431 Cell Line with cellular localization occurring in the nucleus and cytoplasm.
The PTEN antibody is an excellent addition to any research lab. Visit Spring Bioscience to learn more today.
