The P53 antibody is considered a tumor suppressor gene that is expressed in many tissue types and involved in regulating replication, apoptosis and cell growth. It can bind to the SV40T and MDM2 antigens, as well as the human papillomavirus E6 protein. It can sense damage to the DNA and can help with the repair. Mutations involve P53 can be found in many malignant tumors, including ovarian, bladder, breast, colon, melanoma, and lung cancers.
This antibody is designed to be used for research only and has a clone of SPM 514. The immunogen is the recombinant P53 protein in humans. It also has an isotype of IgG20/K with an undetermined epitope. The molecular weight is 53kDa, and it has been tested in humans.
Applications
The P53 antibody can be used with Immunohistochemistry applications and will follow the primary IHC procedure. You will use paraffin-embedded or Formalin-fixed tissues to prepare your specimen. You will also be required to use deparaffinized slides, which must be cleaned with xylene, an alternative to xylene, and graded alcohols.
You can find a pre-diluted formula for this antibody, which can make it easier to use. Otherwise, you can buy concentrated formats and dilute it in-house, using a ratio of 1:400.
The antigen retrieval process requires you to use a 10mM Citrate buffer, boiling the tissue sections for 10 minutes and allowing it to cool to room temperature for at least 20 minutes. The incubation period is 30 minutes at room temperature.
You can rinse the slides between steps using PBS-Tween solutions.
The positive control is the colon carcinoma with a cellular localization in the nucleus.
The P53 antibody can help to repair damage caused by various cancers. If you are testing these antibodies, consider purchasing them from Spring Bioscience today.
