PTEN, also known as Phosphatidylinositol-3, 4, and 5, as well as triphosphate 3-phosphatase, or dual-specificity protein phosphatase is part of the Pl3K/PTEN/Akt pathway and is considered a tumor suppressor. Defects of the PTEN antibody have been showcases in human cancers, including prostate, breast, thyroid, endometrium, skin, neck, brain, and head. Over half of advanced prostate cancer shows a loss of protein expression and abnormal PTEN gene expression.
The PTEN antibody can only be used for research, and it has a clone called SP218. The immunogen is a synthetic peptide located in the C-terminus of the PTEN protein in humans. The IG isotype is the Rabbit IgG, and there are no determined epitopes. It has been tested in humans and has a molecular weight of 54 kDa.
Applications for use include Flow Cytometry, Western Blotting, and Immunohistochemistry. The flow cytometry positive control is the A431 Cell Line with a cellular localization in the nucleus and cytoplasm. You should dilute the antibody using a ratio of 1:200, with an incubation period of 30 minutes at four degrees Celsius.
Western blotting dilution ratios are 1:400, with an incubation period of one hour at room temperature, though dilutions are an estimate. Likewise, the positive control for Western Blotting is the HeLa Cell Lysate.
The IHC positive control is the prostate and prostate adenocarcinoma. The procedure includes using paraffin-embedded or Formalin-fixed tissues and deparaffinized slides. Likewise, dilution should come in the ratio of 1:200, depending on your usage.
Options For Purchase
You can choose smaller amounts and dilute as needed or can choose a pre-diluted form of the PTEN antibody. This allows you to select the most appropriate option based on your particular needs.
The PTEN antibody is an excellent option if you’re researching a variety of cancers. Visit Spring Bioscience now for more information.